Work

CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Year: 2018

Type: article

Abstract: CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.

Source: Nature Communications

DOI: 10.1038/s41467-018-04651-5

Authors: Toon Swings, David C. Marciano, Benu Atri, Rachel E. Bosserman, Chen Wang, Marlies Leysen, Camille Bonte, Thomas Schalck, Ian Furey, Bram Van den Bergh, Natalie Verstraeten, Peter J. Christie, Christophe Herman, Olivier Lichtarge, Jan Michiels

Institutions: VIB-KU Leuven Center for Microbiology, KU Leuven, Baylor College of Medicine

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $6,990

Funders: National Science Foundation, Fonds Wetenschappelijk Onderzoek, KU Leuven, National Institutes of Health, National Institute of General Medical Sciences, Division of Biological Infrastructure

Awards: G1041412011, G129467647, G2147181636, G2224659361, G306787497, In vivo multidrug-tolerant persister cells and their evolution in the face of antibiotic treatment: genetic and physiological adaptation mechanisms, Elucidating the role of two novel toxin-antitoxin modules in Escherichia coli multidrug tolerance., Functional Determinants in G-Protein-Coupled Receptors, G4603166441, G4797008958, ABI Innovation: Towards Recovery of Biological Information, G763275908, In-depth analysis of Pseudomonas aeruginosa persistence mechanisms, G9000007726, G9000114762, G9000217251, G9000241198, G9000267799, G9000318801

OpenAlex ID: W2805453650

FWCI: 0.078

Cites: 32

Cited by: 21

Related to: 10