Year: 1998
Type: dissertation
Abstract: In vitro studies show that FFA impair insulin degradation in rat hepatocytes. To investigate whether FFA impair hepatic insulin extraction in vivo, we infused saline (SAL) or 10% Intralipid (0.03 ml/kg$\cdot$min) + Heparin (0.44 U/kg$\cdot$min) (IH) into 8 normal dogs. An intraportal insulin infusion (INF$\sb{\rm ins}$) was given at 3 mU/kg$\cdot$min for 150 min (period A), at 0.4 mU/kg$\cdot$min (period B) for 150 min and peripherally at 1.4 mU/kg$\cdot$min (period C) for 120 min to assess insulin plasma clearance rate (PCR$\sb{\rm ins}$). FFA were 6-9 fold higher with IH in A, B, and C. Peripheral insulin levels were greater with IH in all periods. PCR$\sb{\rm ins}$ was lower (p $<$ 0.001) with IH (9.7 $\pm$.6 vs 11.5 $\pm$ 0.8 ml/kg$\cdot$min). We calculated the systemic appearance of insulin as (Ra$\sb{\rm sys}$ = PCR$\sb{\rm ins}$x(INS) $\sb{\rm ss}$) and hepatic insulin extraction as (HIE,%) = (INF$\sb{\rm ins}$-Ra$\sb{\rm sys}$)/INF$\sb{\rm ins}$x 100 = (1-(PCR$\sb{\rm ins}$x(INS) $\sb{\rm ss}$)/Inf$\sb{\rm ins}$) x 100. HIE was lower with IH in A (IH = 17.1 $\pm$ 5% vs SAL = 21.3 $\pm$ 3%, p $<$ 0.02), and B (IH = 48.3 $\pm$ 6%, vs SAL = 53.8 $\pm$ 3% p $<$ 0.01). Conclusion. FFA impair hepatic insulin extraction in vivo.
Author: Stephanie R. Wiesenthal
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2805622233