Work

Preparation of Cell Cultures and Vaccinia Virus Stocks

Year: 2015

Type: article

Abstract: The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included.

Source: Current Protocols in Microbiology

DOI: 10.1002/9780471729259.mc14a03s39

Authors: Catherine A. Cotter, Patricia L. Earl, Linda S. Wyatt, Bernard Moss

Institution: National Institute of Allergy and Infectious Diseases

Language: English

Topic: Poxvirus research and outbreaks

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G9000108741