Year: 2018
Type: article
Abstract: Adult schistosomes live in the blood vessels and cannot easily be sampled from humans, so archived miracidia larvae hatched from eggs expelled in feces or urine are commonly used for population genetic studies. Large collections of archived miracidia on FTA cards are now available through the Schistosomiasis Collection at the Natural History Museum (SCAN). Here we describe protocols for whole genome amplification of Schistosoma mansoni and Schistosome haematobium miracidia from these cards, as well as real time PCR quantification of amplified schistosome DNA. We used microgram quantities of DNA obtained for exome capture and sequencing of single miracidia, generating dense polymorphism data across the exome. These methods will facilitate the transition from population genetics, using limited numbers of markers to population genomics using genome-wide marker information, maximising the value of collections such as SCAN.
Source: Parasitology
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,550
Funders: Bill and Melinda Gates Foundation, University of Georgia, Wellcome Trust, Texas Biomedical Research Institute, Georgia Clinical and Translational Science Alliance, Theodor Bilharz Research Institute, European Commission, National Institutes of Health, University of Georgia Research Foundation, Biotechnology and Biological Sciences Research Council, National Institute of Allergy and Infectious Diseases, National Center for Research Resources, National Center for Advancing Translational Sciences
Awards: SCAN: The Schistosomiasis Collections at the Natural History Museum., G1173815590, G140538419, G1560697804, G2169778925, G2361851875, Structure-guided redesign of an antischistosomal drug, Genetic Basis of Praziquantel Resistance, G305836430, G3072562321, Institute for Integration of Medicine & Science: A Partnership to Improve Health, G3692875803, EXTRAMULAR RESEARCH FACILITIES CONSTRUCTION, G4326864042, SCAN: The Schistosomiasis Collections at the Natural History Museum., G518778165, Genetic analysis of cercarial release in schistosomes, G5947346353, G6253890364, MOLECULAR AND BIOCHEMICAL GENETICS LABORATORY RENOVATION, G7249703675, Genetic Analysis of Host Specificity in Schistosoma mansoni, G7394745119, The interplay of wildlife, livestock and humans in African zoonotic-schistosomiasis transmission dynamics, Institute for Integration of Medicine & Science: A Partnership to Improve Health, Epidemiology and evolution of zoonotic schistosomiasis in a changing world, Efficient Linkage Mapping Methods for Schistosoma mansoni
OpenAlex ID: W2805938446