Work

Comparison of illumination geometry for lifetime‐based measurements in whole‐body preclinical imaging

Year: 2018

Type: article

Abstract: Macroscopic fluorescence lifetime imaging (MFLI) has been proved to be an accurate tool to quantify Förster resonance energy transfer (FRET) lifetime-based assessment of receptor-ligand engagement in vitro and in vivo. Herein, we report on the quantitative comparison of MFLI for whole-body preclinical studies in transmittance and reflectance geometries. The comparative study was conducted for both in vitro and in vivo conditions. FRET quantification performance in both geometries was similar in high fluorescence concentration samples. However, the reflectance geometry performed better at low fluorescence concentration. In addition, reflectance geometry could acquire subsurface imaging of the main whole-body organs of small animals without being compromised by tissue attenuation.

Source: Journal of Biophotonics

DOI: 10.1002/jbio.201800037

Authors: Nattawut Sinsuebphon, Alena Rudkouskaya, Margarida Barroso, Xavier Intes

Institutions: Rensselaer Polytechnic Institute, Albany Medical College

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering