Work

Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation

Year: 2018

Type: article

Abstract: OBJECTIVE: This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay. RESULTS: We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay.

Source: BMC Research Notes

DOI: 10.1186/s13104-018-3455-x

Authors: Ira M. Sigar, Justin H. Schripsema, Kathleen A. Kelly, Ashlesh K. Murthy, Srikanth Manam, Kyle H. Ramsey

Institutions: Midwestern University, University of California, Los Angeles

Language: English

Topic: Microbial infections and disease research

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,690