Work

Image-Based Analysis of Mitochondrial Area and Counting from Adult Mouse Dopaminergic Neurites

Year: 2018

Type: article

Abstract: Mitochondria form dynamic cytoplasmic networks which undergo morphological changes in order to adapt to cellular stresses and signals. These changes can include alterations in size and number within a given cell. Analysis of the whole network can be a useful metric to assess overall mitochondrial health, particularly in neurons, which are highly sensitive to mitochondrial dysfunction. Here we describe a method which combines immunofluorescence and computerized image analysis to measure mitochondrial morphology (quantification of number, density, and area) in dopaminergic neurites of mice expressing mitochondrially-targeted eYFP.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.2471

Authors: Nadee Nissanka, Carlos Moraes, Milena Pinto

Institution: University of Miami

Language: English

Topic: Mitochondrial Function and Pathology

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institute on Aging, National Eye Institute, National Institute of Neurological Disorders and Stroke