Respiratory Syncytial Virus Fusion Protein Interaction with its Cellular Receptor, Nucleolin
Year: 2018
Type: dissertation
Abstract: Respiratory Syncytial virus (RSV) fusion protein (F) interacts with nucleolin during viral fusion. RNA binding domains 1 and 2 (RBDs) of nucleolin, when expressed as a complex, inhibits RSV infection in vitro. We examined the interaction between pre- vs. post-fusion F, and whole virus, with the RBDs complexed and individually. Both RSV Fs co-precipitated RBD1,2. Immobilized RBD1,2 precipitated both RSV Fs and whole viral particles. Immobilized RBD1 precipitated post-fusion but not pre-fusion RSV F. RBD1,2 inhibited infection at 20nM when co-incubated with the virus and the cells for 24 hours. RBD1 inhibited infection to a lesser extent with the same conditions. RBDs were not able to inhibit infection with coincubation with virus prior to infection. RBD1,2 interacts with RSV F, showing different preferential binding for one conformation over another depending on assay conditions, while inhibiting infection at 20nM with 24 hours of coincubation.
Source: TSpace (University of Toronto)
Author: Daniel Zheng Yuan Zhu
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2806847805