Lineage tracing of murine adult hematopoietic stem cells reveals active contribution to steady-state hematopoiesis
Year: 2018
Type: article
Abstract: Characterization of hematopoietic stem cells (HSCs) has advanced largely owing to transplantation assays, in which the developmental potential of HSCs is assessed generally in nonhomeostatic conditions. These studies established that adult HSCs extensively contribute to multilineage hematopoietic regeneration upon transplantation. On the contrary, recent studies performing lineage tracing of HSCs under homeostatic conditions have shown that adult HSCs may contribute far less to steady-state hematopoiesis than would be anticipated based on transplantation assays. Here, we used 2 independent HSC-lineage-tracing models to examine the contribution of adult HSCs to steady-state hematopoiesis. We show that adult HSCs contribute robustly to steady-state hematopoiesis, exhibiting faster efflux toward the myeloid lineages compared with lymphoid lineages. Platelets were robustly labeled by HSCs, reaching the same level of labeling as HSCs by 1 year of chase. Our results support the view that adult HSCs contribute to the continuous influx of blood cells during steady-state hematopoiesis.
Source: Blood Advances
Institution: Baylor College of Medicine
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,800
Awards: Baylor-UT Houston Center for AIDS Research, Mesenchymal Stromal Cells Regulate Hematopoietic Stem Cell Aging, BD Biosciences Special Order LSRII, Hematology Training Program, Program in Human and Molecular Genetics, METABOLIC REGULATION IN LEUKEMIA-INITIATING CELLS, Baylor College of Medicine Cancer Center - Cancer Center Support Grant
OpenAlex ID: W2807354979