Year: 2018
Type: article
Abstract: Leukocyte adhesion and extravasation are maximal near the transition from capillary to post-capillary venule, and are strongly influenced by a confluence of scale-dependent physical effects. Mimicking the scale of physiological vessels using in vitro microfluidic systems allows the capture of these effects on leukocyte adhesion assays, but imposes practical limits on reproducibility and reliable quantification. Here we present a microfluidic platform that provides multiple (54-512) technical replicates within a 15-minute sample collection time, coupled with an automated computer vision analysis pipeline that captures leukocyte adhesion probabilities as a function of shear and extensional stresses. We report that in post-capillary channels of physiological scale, efficient leukocyte adhesion requires erythrocytes forcing leukocytes against the wall, a phenomenon that is promoted by the transitional flow in post-capillary venule expansions and dependent on the adhesion molecule ICAM-1.
Source: Scientific Reports
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,690
Funders: Biogen, Case Western Reserve University, Case Comprehensive Cancer Center, Case Western Reserve University, National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering, National Center for Research Resources, National Center for Advancing Translational Sciences
Awards: G1351460746, G1361233939, G1436032867, G1441372350, G156741193, G27948943, G3681549450, Standardized Monitoring of Cellular Adhesion to Improve Clinical Care in Sickle Cell Disease, Using Microfluidics to Investigate Mechanisms of Leukocyte Intravascular Crawling, G4516325736, Cdk5 signaling in ICN1-induced T Cell Leukemogenesis, G7224416095, G9000012232, G9000066886, G9000092448, G9000286568, G9000337204, Case Comprehensive Cancer Center Support Grant, Immunology Training Program-Predoctoral
OpenAlex ID: W2807801504