An acoustofluidic trap and transfer approach for organizing a high density single cell array
Year: 2018
Type: article
Abstract: We demonstrate a hybrid microfluidic system that combines fluidic trapping and acoustic switching to organize an array of single cells at high density. The fluidic trapping step is achieved by balancing the hydrodynamic resistances of three parallel channel segments forming a microfluidic trifurcation, the purpose of which was to capture single cells in a high-density array. Next, the cells were transferred into adjacent larger compartments by generating an array of streaming micro-vortices to move the cells to the desired streamlines in a massively parallel format. This approach can compartmentalize single cells with efficiencies of ≈67% in compartments that have diameters on the order of ∼100 um, which is an appropriate size for single cell proliferation studies and other single cell biochemical measurements.
Source: Lab on a Chip
DOI: 10.1039/c8lc00196k
Authors: Korine A. Ohiri, Sean T. Kelly, Jeffrey D. Motschman, Kevin H. Lin, Kris C. Wood, Benjamin B. Yellen
Institutions: U.S. National Science Foundation, Duke University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
Awards: Triangle Center for Excellence for Materials Research and Innovation: Programmable Assembly of Soft Matter, G1511584654, G1810985955, Medical Scientist Training Program, G3870562522, G4143183676, GRADUATE RESEARCH FELLOWSHIP PROGRAM, NNCI: North Carolina Research Triangle Nanotechnology Network (RTNN), Magnetically directed single cell transcriptome analysis in HIV latency, G9000067156, G9000135944
OpenAlex ID: W2808792789