Fingerprint Stimulated Raman Scattering Imaging Reveals Retinoid Coupling Lipid Metabolism and Survival
Year: 2018
Type: article
Abstract: Retinoids play critical roles in development, immunity, and lipid metabolism, and their deficiency leads to various human disorders. Yet, tools for sensing retinoids in vivo are lacking, which limits the understanding of retinoid distribution, dynamics and functions in living organisms. Here, using hyperspectral stimulated Raman scattering microscopy, we discover a previously unknown cytoplasmic store of retinoids in Caenorahbditis elegans. Following the temporal dynamics of retinoids, we reveal that their levels are positively correlated with fat storage, and their supplementation slows down fat loss during starvation. We also discover that retinoids promote fat unsaturation in response to high-glucose stress, and improve organism survival. Together, our studies report a new method for tracking the spatiotemporal dynamics of retinoids in living organisms, and suggest the crucial roles of retinoids in maintaining metabolic homeostasis and enhancing organism fitness upon developmental and dietary stresses.
Source: ChemPhysChem
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
Funders: Howard Hughes Medical Institute, National Institutes of Health, National Institute on Aging, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Biomedical Imaging and Bioengineering, National Center for Complementary and Integrative Health
Awards: Delineate the regulatory network of lipid metabolism via SRS imaging-sorting, G2053725305, G2137056809, G5905248492, High-throughput high-content single cell analysis by multichannel stimulatedRaman flow cytometry, Microsecond Raman spectroscopy: Assessing single cell metabolism in a vital organism, G9000020420, G9000089372, G9000191992, G9000203969, G9000214629
OpenAlex ID: W2808913111