Year: 2018
Type: preprint
Abstract: Here we present preliminary results for a method of oligodeoxynucleotide synthesis using terminal deoxynucleotidyl transferase (TdT) to generate mixed base homopolymer runs of defined sequence. In a process we have termed Free-Running Synthesis (FRS), we allow TdT to freely add multiple bases of a given monomer. With this method, homopolymeric runs can be used for writing DNA in a data storage capacity, where a stretch of A’s, for example, will be read as a single base, and the transitions between successive homopolymer runs encodes information. As a proof-of-concept, we demonstrate homopolymer additions of A, C and T onto the 3’ end of a 22 base initiator strand.
DOI: 10.1101/355719
Authors: M.A. Jensen, P.B. Griffin, R. W. Davis
Institution: Stanford University
Language: English
Topic: DNA and Biological Computing
Subfield: Theoretical Computer Science
Field: Mathematics
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2810310556