Get started
Work

Molecular screening for Neisseria gonorrhoeae antimicrobial resistance markers in Nigerian men who have sex with men and transgender women

Year: 2018
Type: article
Study design: Observational Study
Abstract: Antimicrobial-resistant Neisseria gonorrhoeae (NG) is a global public health issue that threatens effectiveness of current treatments of NG. Increased use of nucleic acid amplification tests (NAATs) in lieu of cultures makes obtaining clinical isolates for susceptibility testing difficult and samples collected in commercial transport buffer for NAATs do not preserve viable organism, while molecular methods of assessing antibiotic susceptibility do not require viable organism. We evaluated 243 NG-positive samples in Aptima transport media including urine, oral, and rectal swabs from Nigerian men who have sex with men for markers to penicillinase-producing NG, ciprofloxacin ( GyrA and ParC mutations), and extended spectrum cephalosporins (ESCs, PenA mosaic [allele X], PonA, mtrR, PorB mutations) by real-time PCR. NG DNA was recovered in 75% (183/243) of samples. Of these, 93% (171/183) were positive for at least one resistance marker. We observed a prevalence of dual resistance markers to penicillin and ciprofloxacin at 46.2% (79/171). Six percent of samples (10/171) tested positive for the PenA mosaic (allele X) ESC marker. These data indicate that antibiotic-resistant NG is common in Nigeria. Laboratory and clinical capacity building in Nigeria should include development of methods to culture NG and determine antimicrobial susceptibility.
DOI: 10.1177/0956462418780050
Authors: Justin Hardick, Trevor A Crowell, Kara Lombardi, Akindiran Akintunde, Sunday Odeyemi, Andrew Ivo, George Eluwa, Jean Njab, Stefan D Baral, Rebecca G Nowak, Thomas C Quinn, Kent Barbian, Sarah Anzick, Sylvia Adebajo, Manhattan E Charurat, Julie Ake, Charlotte A Gaydos
Institutions: Johns Hopkins University, Henry M. Jackson Foundation, Walter Reed Army Institute of Research, University of Maryland, Baltimore, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories
Language: English
Open Access status: green
Funders: U.S. President’s Emergency Plan for AIDS Relief, National Institutes of Health, National Institute of Mental Health, National Institute of Allergy and Infectious Diseases, Fogarty International Center, National Institute of Biomedical Imaging and Bioengineering
Awards: Technology development for point-of-care detection and antimicrobial susceptibility testing of Neisseria gonorrhoeae, TRUST, UM-IHV AITRP in Nigeria, Building TRUST, Center for Innovative Diagnostics for Infectious Diseases, LC: HIV PREVENTION TRIALS NETWORK, International Studies of the Acquired Immune Deficiency Syndrome (AIDS)
OpenAlex ID: W2810479363
FWCI: 0.472
Cites: 35
Cited by: 9
Related to: 10