A method for single-neuron chronic recording from the retina in awake mice
Year: 2018
Type: article
Abstract: The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.
Source: Science
Institutions: Harvard University, Korea University
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
Funders: Howard Hughes Medical Institute, American Heart Association, National Institute on Aging, NIH Office of the Director, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering, NIH Blueprint for Neuroscience Research, Air Force Office of Scientific Research
Awards: Synaptic Choices in the Retinotectal System, Brain Aging Studies with Single-Neuron Resolution Using Syringe-Injectable Electronics, G2111980146, G3054726632, G4035565721, Brain Aging Studies with Single-Neuron Resolution Using Syringe-Injectable Electronics, Syringe-Injectable Electronics for Neurological Investigation and Medical Treatment of Stroke, Syringe-Injectable Mesh Electronics for Seamless Integration with the Central Nervous System
OpenAlex ID: W2811445844