Work

High-throughput screening of biomolecules using cell-free gene expression systems

Year: 2018

Type: review

Abstract: The incorporation of cell-free transcription and translation systems into high-throughput screening applications enables the in situ and on-demand expression of peptides and proteins. Coupled with modern microfluidic technology, the cell-free methods allow the screening, directed evolution and selection of desired biomolecules in minimal volumes within a short timescale. Cell-free high-throughput screening applications are classified broadly into in vitro display and on-chip technologies. In this review, we outline the development of cell-free high-throughput screening methods. We further discuss operating principles and representative applications of each screening method. The cell-free high-throughput screening methods may be advanced by the future development of new cell-free systems, miniaturization approaches, and automation technologies.

Source: Synthetic Biology

DOI: 10.1093/synbio/ysy012

Authors: Luis E Contreras-Llano, Cheemeng Tan

Institution: University of California, Davis

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,250

Funders: University of California Institute for Mexico and the United States, Human Frontier Science Program, Branco Weiss Fellowship – Society in Science, Consejo Nacional de Ciencia y Tecnología