Investigation of molecular recognition in biological systems using cellular membrane affinity chromatography.
Year: 2008
Type: article
Abstract: Cellular membrane affinity chromatography (CMAC) columns have been created through the immobilization of cellular membrane fragments on liquid chromatographic supports. A CMAC column containing the human organic cation transporter, CMAC(hOCT1) column, has been used to study the stereoselective binding of competitive inhibitors. The chromatographic data obtained using the CMAC(hOCT1) column was to develop a pharmacophore model that described the stereoselectivity. The results indicate that a dynamic chiral recognition model based upon conformational adjustments between the inhibitors and hOCT1 is responsible for the observed steroeselectivity.
Source: PubMed
Author: Irving W Wainer
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Funder: National Institute on Aging
OpenAlex ID: W28235251