Work

In Vitro Models for Studying Trophoblast Transcellular Transport

Year: 2006

Type: book-chapter

Abstract: In vitro models have proven to be effective in studying the placental transporters that play a role in the exchange of nutrients, waste products, and drugs between the maternal and fetal circulations. Although primary cultures of trophoblast cells can be used to perform uptake, efflux, and metabolism studies, only the rodent HRP-1 and the human BeWo cell lines have been shown to form confluent monolayers when grown on semi-permeable membranes. Protocols for the revival, maintenance, passage, and growth of BeWo cells for transporter expression and transcellular transport studies are provided.

Source: Humana Press eBooks

DOI: 10.1385/1-59259-989-3:225

Authors: Claudia J. Bode, Hong Jin, Erik Rytting, Peter S. Silverstein, Amber M. Young, Kenneth L. Audus

Institution: University of Kansas

Language: English

Topic: Drug Transport and Resistance Mechanisms

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Institute of Child Health and Human Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development