Micropropagation of sour cherry Čačanski rubin' (Prunus cerasus L.).
Year: 2013
Type: article
Abstract: The paper describes the protocol for micropropagation of sour cherry 'Čačanski Rubin' (Prunus cerasus L.) bred at the Fruit Research Institute in Čačak. Aiming at developing efficient protocol for micropropagation of this genotype, the influence of repeated subculturing on proliferation capacity of in vitro shoots as well as the effect of change of type and concentration of plant growth regulators on multiplication and rooting phases were studied. With standard method of surface sterilization of initial explants, aseptic culture was established and leaf rosettes were initiated on Murashige and Skoog (1962) medium (MS) with 2.0 mg l-1 BAP, 0.5 mg l-1 IBA and 0.1 mg l-1 GA3. After establishment of aseptic culture, regenerated shoots were multiplied in five successive subcultures on MS medium supplemented with 0.5 mg l-1 BAP in combination with IBA and GA3 each applied at 0.1 mg l-1. The following multiplication parameters were monitored: multiplication index, length of axial and lateral shoots. With aim to optimize multiplication stage and determine the optimal hormonal composition of multiplication medium, the influence of increase in BAP concentration and/or type of auxins (IBA or NAA), on multiplication index, fresh and dry weight of shoots was examined in fifth subculture after initiation. During multiplication on MS medium with constant hormonal composition, the decline in shoot formation capacity over repeated subcultures was observed, so as the highest value of multiplication index (3.11) was noticed in first subculture after initiation. However the subculturing improved the shoot elongation, and the highest value of shoots length, especially in axial shoots (1.22 cm), was observed in fifth subculture. Although increase in BAP concentration from 0.5 to 1.0 mg l-1 in combination with NAA instead of IBA in fifth subculture increased multiplication index, the shoots multiplied on this medium were not suitable for further growing due to the significant decrease in shoot length as well as to the appearance of hyperhydricity. Sour cherry 'Čačanski Rubin' displayed very low rooting ability on HF medium as well as on medium with 1.0 mg l-1 IBA which is the most common medium used for rooting of different species of Prunus genus, while on medium containing NAA at same concentration rhizogenesis was not observed. The highest rooting rate (60.0%) as well as the highest number of roots per shoot (6.3) in this genotype were achieved with 1-min dip treatment in NAA dissolved in sterile water (500 mg l-1) followed by growing on HF medium. The percentage of acclimatization under the 'mist' system in green house was significantly higher for shoots rooted in vitro (55.6%) than for unrooted shoots (14,8%).
Authors: T. Vujović, Đ. Ružić, Robert Cerović
Language: Croatian
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W284275863