The effect of Rosmarinic acid on glycine betaine, carbohydrate and protein pattern changes of potato (Solanum tuberosum L.) callus under in vitro condition
Year: 2018
Type: article
Abstract: Salt stress is one of the most destructive abiotic stress which affects compatible osmolytes and protein pattern. In the current study, the effect of rosmarinic acid as a polyphenolic quencher on compatible solutes such as glycine betaine, total carbohydrates and protein, Na+ and K+ and fresh weight were determined to evaluate osmotic adjustment as a stress tolerance mechanism in Solanum tuberosum L. c.v. White Desire in vitro condition. In the other experiments, SDS-PAGE was performed to verify feasible hypothesis of ameliorative effects of RA on biosynthesis of proteins. In vitro-grown cells of potato were incubated on Murashige and Skoog (MS) medium containing 0, 20, 40, and 60 mg/L RA and 0, 90, and 120 mM NaCl. Data showed increase in fresh weight in 120 mM-stress calli pretreated with rosmarinic acid compare to 120 mM stressed calli. Carbohyadrate content increased notably by using RA in exposure to NaCl. The results showed a significant increase in glycine betaine in 90 mM NaCl +20 mg/L RA treated calli compared with 90 mM NaCl-stressed calli. There was a notable increase in contents of Na+ and decline in K+ content in in vitro calli cells grown under 90 and 120 mM salt levels. Interestingly, K+ contents in RA-treated calli cells were remarkably higher than Na+ contents. However, no pronounced changes in K+/ Na+ ratio were observed. Data showed that in vitro RA-treated calli responded rapidly to external RA supplement showing higher proteins contents compared to controls. SDS-PAGE data indicated a positive impact of RA on protein pattern. In vitro calli cells treated with 20 and 60 mg/l RA showed the induction of 2 new protein bands with approximately 50 KD while, 3 protein bands with approximately 16, 17 and 25 KD were down-regulated by RA. Consequently, data presented in the current study indicated a positive link between salt tolerance and improved metabolites biosynthesis in potato White Desire following RA treatment.
Authors: Hoda Eskandari, Ali Akbar Ehsanpour, Naemeh Al Mansour
Institutions: University of Isfahan, Kuwait University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2883316652