Establishing a new rodent model of partial bladder outlet obstruction and the role of macrophages in bladder obstruction
Year: 2017
Type: dissertation
Abstract: Partial bladder outlet obstruction has a high prevalence, develops insidiously, and can severely affect quality of life and health of patients. Obstruction leads to bladder hypertrophy and ultimately to fibrosis. While macrophages are known as central regulators of fibrosis in many organs, their role in bladder remodeling is poorly understood. We hypothesized that macrophages modulate bladder remodeling in the context of partial outlet obstruction. We developed a partial bladder outlet obstruction rodent model that avoids dissection around the bladder neck, thus avoiding a wound healing response of that area affecting the bladder. Importantly, our model also preserved bladder innervation, in contrast to the traditionally used model. Bladder outlet obstruction increased the number of macrophages and depletion of macrophages not only affected cytokine levels in bladder tissue but also helped preserve bladder function. Macrophages seem to contribute to obstruction-related bladder dysfunction
Source: TSpace (University of Toronto)
Author: Martin Sidler
Language: English
Subfield: Urology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2883331284