Preformed Vascular Networks Survive and Enhance Vascularization in Critical Sized Cranial Defects
Year: 2018
Type: article
Abstract: Vascular networks provide nutrients, oxygen, and progenitor cells that are essential for bone function. It has been proposed that a preformed vascular network may enhance the performance of engineered bone. In this study vascular networks were generated from human umbilical vein endothelial cell and mesenchymal stem cell spheroids encapsulated in fibrin scaffolds, and the stability of preformed vascular networks and their effect on bone regeneration were assessed in an in vivo bone model. Under optimized culture conditions, extensive vessel-like networks formed throughout the scaffolds in vitro. After vascular network formation, the vascularized scaffolds were implanted in a critical sized calvarial defect in nude rats. Immunohistochemical staining for CD31 showed that the preformed vascular networks survived and anastomosed with host tissue within 1 week of implantation. The prevascularized scaffolds enhanced overall vascularization after 1 and 4 weeks. Early bone formation around the perimeter of the defect area was visible in X-ray images of samples after 4 weeks. Prevascularized scaffolds may be a promising strategy for engineering vascularized bone.
Source: Tissue Engineering Part A
Authors: Brianna M. Roux, Banu Akar, Wei Zhou, Katerina Stojkova, Beatriz Barrera, Jovan Brankov, Eric M. Brey
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
Funders: National Science Foundation, U.S. Department of Veterans Affairs, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Biomedical Imaging and Bioengineering, Division of Chemical, Bioengineering, Environmental, and Transport Systems
Awards: An Enabling Technology for Preclinical X-Ray Imaging of Biomaterials In-Vivo, G1313251248, Analyzer-based Phase Contrast Imaging System Development and Evaluation, CDI-Type II: Optimization of Engineered Tissue Growth by Active Learning, New Class of Numerical Observers for Nuclear Cardiology, Collaborative Research: Development of Preclinical X-ray Phase-Contrast Tomography for 3D Imaging of Engineered Tissues, Guided Tissue Engineering of 3D Vascularized Tissues, G9000005856
OpenAlex ID: W2883475305