Improved protocol for spawning and immunostaining embryos and juvenile stages of the ctenophore Mnemiopsis leidyi
Year: 2018
Type: article
Abstract: Ctenophores are becoming of increasing interest to researchers interested in cellular and developmental evolutionary biology.One species, _Mnemiopsis leidyi_, has been one of the most heavily studied ctenophores due to its availability and because it was the rst ctenophore to have its genome sequenced and made publicly available.However, their gelatinous and delicate consistency makes experimental handling challenging and time-consuming.Here we describe improvements for scheduling adult spawning and new handling techniques for further histological and molecular analyses.We hope that this contribution will establish an opportunity for other workers to improve these methods and allow a rapid increase in our understanding of ctenophore biology.
Source: Protocol Exchange
Language: English
Subfield: Paleontology
Field: Earth and Planetary Sciences
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W2883476628