Work

Soluble Expression of Humanized Anti-CD20 Single Chain Antibody in Escherichia coli by Cytoplasmic Chaperones Co-expression.

Year: 2018

Type: article

Abstract: BACKGROUND: . METHODS: BL21 (DE3) was evaluated. In order to inhibit the production of IBs, the effects of co-expression with cytoplasmic chaperones GroEL, DnaK, GroES, Tig, DnaJ and GrpE were investigated. RESULT: soluble anti-CD20 scFv. CONCLUSION: .

Source: Europe PMC (PubMed Central)

Authors: Mohammadreza Yousefi, Safar Farajnia, Ahad Mokhtarzadeh, Bahman Akbari, Shiva Ahdi Khosroshahi, Mina Mamipour, Hassan Dariushnejad, Vahideh Ahmadzadeh

Institutions: Tabriz University of Medical Sciences, Gonabad University of Medical Sciences, Kermanshah University of Medical Sciences, Biotechnology Research Center

Language: English

Topic: Protein purification and stability

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2884362210

FWCI: 0.468

Cites: 24

Cited by: 11

Related to: 10