Work

Reply to ‘Trace N-glycans including sulphated species may originate from various plasma glycoproteins and not necessarily IgG’

Year: 2018

Type: letter

Abstract: The correspondence from Lauc et al. 1 argues that a high level of H5N4S2 (abbreviated as Hex5HexNAc4NeuAc2 in our original article 2 , which has a combined relative abundance of 5.4 %) indicates contamination of other plasma glycoproteins in our immunoglobulin G (IgG) samples. Therefore, they speculate that many trace glycans identified in our study originated from other plasma glycoproteins, but not IgG. We are thankful for the interest in our work; however, we do not agree with this conclusion as it is inferred from a generalization of a “relative abundance-based indicator”, incorrect interpretation of our data, and more importantly, incomplete consideration of the intrinsic high sensitivity and specially improved detection sensitivity for acidic glycans of our method. Herein, we address the query point by point and provide additional experimental results in support of our published article.

Source: Nature Communications

DOI: 10.1038/s41467-018-05082-y