Year: 2017
Type: article
Abstract: Enteric coronaviruses (CoVs) of pigs include transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV) and porcine delta coronavirus (PDCoV).They cause significant economic losses in pig industry worldwide.In the US, PEDV emerged in 2013 and caused the death of over 7 million piglets within the first year.Feral pigs have contributed to the transmission of different viral, bacterial and parasitic infections to domestic pigs, other domestic animals and even to humans.However, their role in maintenance and transmission of porcine enteric CoVs is not well understood.In this study, fecal samples of 44 feral pigs were collected from California, USA in earlymid 2016.RNA was extracted from the fecal content supernatant and subjected to conventional reverse transcription (RT)-PCR using two different panCoV primers to detect the presence or absence of porcine enteric CoVs.Quantitative RT-PCR (RT-qPCR) using PEDV N gene and PDCoV M gene specific primers and probes were also performed for amplification of specific viral genes.DNA sequencing of PCR products from likely positive sample was also performed for further confirmation.None of the samples were clearly positive using conventional RT-PCR assays.Few samples were doubtful for PEDV or PDCoV genes and showed Ct values slightly above the detection limit.Repeated testing and/or DNA sequencing confirmed that those samples were negative for PEDV or PDCoV presence.Our results indicated that no iii porcine enteric CoVs were circulating in the feral pigs in California in early-mid 2016.Similar studies from other states of USA with larger sample sizes will be helpful to ascertain whether wild pigs in USA are playing a role in the maintenance and transmission of porcine enteric CoV or not.for her sustained encouragement, regular guidance, inspiration, valuable suggestions and meticulous supervision throughout the study period.My humble thanks go to my advisory committee members Dr. Renukaradhya Gourapura and Dr.
Author: Shristi Ghimire
Institution: The Ohio State University
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2884790602