Work

Prospective Use of High-Refractive Index Materials for Single Molecule Detection in Flow Cytometry

Year: 2018

Type: article

Abstract: Phenotyping extracellular vesicles (EVs), where surface receptor expression is often as low as one molecule per EV, remains problematic due to the inability of commercial flow cytometers to provide single-fluorescent molecule sensitivity. While EVs are widely considered to be of great potential as diagnostic, prognostic and theranostic biomarkers, their use is currently hindered by the lack of tools available to accurately and reproducibly enumerate and phenotype them. Herein, we propose a new class of labels that leverage the biophysical properties of materials with unique complex refractive indices and demonstrate that this class of labels has the possibility of allowing single-epitope detection using conventional flow cytometry.

Source: Sensors

DOI: 10.3390/s18082461

Authors: Joshua A. Welsh, Julia Kepley, Ariel Rosner, Peter Horak, Jay A. Berzofsky, Jennifer C. Jones

Institutions: National Institutes of Health, Center for Cancer Research, University of Southampton

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,137

Funder: National Institutes of Health