Work

Encapsulation of mesenchymal stem cells in glycosaminoglycans‐chitosan polyelectrolyte microcapsules using electrospraying technique: Investigating capsule morphology and cell viability

Year: 2018

Type: article

Abstract: Polyelectrolyte microcapsules are modular constructs which facilitate cell handling and assembly of cell-based tissue constructs. In this study, an electrospray (ES) encapsulation apparatus was developed for the encapsulation of mesenchymal stem cells (MSCs). Ionic complexation between glycosaminoglycans (GAGs) and chitosan formed a polyelectrolyte complex membrane at the interface. To optimize the capsules, the effect of voltage, needle size and GAG formulation on capsule size were investigated. It was observed that by increasing the voltage and decreasing the needle size, the capsule size would decrease but at voltages above 12 kV, capsule size distribution broadened significantly which yields lower circularity. Increase in GAG viscosity resulted in larger microcapsules and cell viability exhibited no significant changes during the encapsulation procedure. These results suggest that ES is a highly efficient, and scalable approach to the encapsulation of MSCs for subsequent use in bioprinting and other modular tissue engineering or regenerative medicine applications.

Source: Bioengineering & Translational Medicine

DOI: 10.1002/btm2.10111

Authors: Amin Vossoughi, Howard W. T. Matthew

Institution: Wayne State University

Language: English

Topic: Electrohydrodynamics and Fluid Dynamics

Subfield: Electrical and Electronic Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,890