Evaluation of Artesunate and Praziquantel Combination Therapy in Murine Schistosomiasis mansoni.
Year: 2018
Type: article
Abstract: BACKGROUND: infection, and study the role of apoptosis in evaluation of the drugs efficacy. METHODS: Eighty laboratory-bred Swiss albino male mice were classified into four groups (20 mice each); control, PZQ treated (500 mg/kg), AS treated (400 mg/kg) and combined AS (400 mg/kg) + PZQ (500 mg/g) groups. Efficacy of the drugs was assessed by parasitological (egg count/gram stool, worm burden, tissue egg load, oogram pattern), histopathological (haematoxylin and eosin -for detection of type of hepatic granulomas, number & diameter) and immunohistochemical studies (P53 and Bcl-2 markers for determination of inflammatory cells and the degree of apoptosis). RESULTS: Significant reduction was recorded in stool egg count, tissue egg count (liver and intestine), worm burden, granuloma number and size and changed oogram patterns in artesunate -praziquantel combined group followed by artesunate monotherapy group. There was a significant increase in the apoptotic proteins P53 and slight increase in anti-apoptotic proteins Bcl-2 in the infected group compared to the control healthy group. A significant decrease and increase in P53 & Bcl-2 expressions respectively were observed in artesunate - praziquantel combined group compared to control infected group. CONCLUSION: apoptosis, morbidity and chemotherapy efficacy.
Source: Europe PMC (PubMed Central)
Authors: Laila Abdel Moniem Hegazy, Mona Hussein Al Motiam, Naglaa Fathy Abd El-Aal, Shereen Mahmoud Ibrahim, Heba Khalil Mohamed
Institutions: Zagazig University, Theodor Bilharz Research Institute
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2886956611