Work

Synthetic cry1AcF Gene Imparts Resistance to Spodoptera litura (F.) and Amsacta albistriga Walker in Peanut (Arachis hypogaea L.) cv. VRI-2

Year: 2018

Type: article

Abstract: Transgenic VRI-2 peanut plants were generated by following an Agrobacterium mediated, in planta transformation protocol with cry1AcF gene for efficacy against Spodoptera litura and Amsacta albistriga. Putative transgenics were selected by analyzing composite samples by GRID PCR method. The transgenic nature of the putative transgenics was further ascertained by molecular analysis for the presence of the gene of interest and selectable marker followed by efficacy against S. litura and/or Amsacta albistriga. The plants that showed 57.9-100 % mortality against S. litura were selected for further analysis. The stability of the selected T1 plants in the subsequent generation was confirmed by not only integration and efficacy but also expression by ELISA. Further, Southern analysis of T2 generation plants revealed the integration pattern of the transgene in the peanut genome and improved larval mortality in the range of 25-78.94%. The study demonstrates that overexpression of cry1AcF in peanut provide resistance to S. litura and A. albistriga.

Source: International Journal of Current Microbiology and Applied Sciences

DOI: 10.20546/ijcmas.2018.708.264

Authors: G. Keshavareddy, A.R.V. Kumar, Vemanna S. Ramu, Rohini Sreevathsa, M. Udayakumar

Institutions: University of Agricultural Sciences, Bangalore, National Research Centre on Plant Biotechnology

Language: English

Topic: Insect Resistance and Genetics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

Funders: National Research Centre, Department of Biotechnology, Ministry of Science and Technology, India