Work

Transposase-Mediated Chromosomal Integration of Exogenous Genes in Acidithiobacillus ferrooxidans

Year: 2018

Type: article

Abstract: Acidithiobacillus ferrooxidans is an iron- and sulfur-oxidizing chemolithoautotroph and is a key member of the microbial consortia used in industrial biomining applications. There is interest in exploiting these cells for other metal recovery applications as well as in developing them as unique nonmodel microbial cell factories. Plasmid-driven expression of exogenous genes has been reported, and homologous recombination has been used to knock out some gene expression. Here, new selection protocols facilitated the development of a transposition method for chromosomal integration of exogenous genes into A. ferrooxidans . This greatly expands the available genetic toolbox, which will open the door to greater metabolic engineering efforts for these cells.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.01381-18

Authors: Yuta Inaba, Indrani Banerjee, Timothy Kernan, Scott Banta

Institution: Columbia University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Science Foundation

Award: SusChEM: Long chain hydrocarbons from CO2 and electricity via genetic modification of a chemolithoautotrophic bacterium