Single-particle cryo-EM—How did it get here and where will it go
Year: 2018
Type: review
Abstract: Cryo-electron microscopy, or simply cryo-EM, refers mainly to three very different yet closely related techniques: electron crystallography, single-particle cryo-EM, and electron cryotomography. In the past few years, single-particle cryo-EM in particular has triggered a revolution in structural biology and has become a newly dominant discipline. This Review examines the fascinating story of its start and evolution over the past 40-plus years, delves into how and why the recent technological advances have been so groundbreaking, and briefly considers where the technique may be headed in the future.
Source: Science
Author: Yifan Cheng
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: green
Awards: Mechanism of gate-opening in the 20S proteasome induced by the proteasomal ATPase, HARC Center: HIV Accessory and Regulatory Complexes, Acquisition of an electron microscope for high-resolution single particle cryo-EM, Mapping the conformational cycle of transmembrane transporters, Structural mechanism of integrin-mediated TGF-b activation, Linux cluster for near atomic resolution single particle cryo-EM, Structures and gating mechanisms of TRP ion channels
OpenAlex ID: W2889085087