Work

Proteomics dataset containing proteins that obscure identification of TOPLESS interactors in Arabidopsis

Year: 2018

Type: data-paper

Abstract: . We generated transgenic plants harboring TPL fused to the GS-TAG, "Boosting tandem affinity purification of plant protein complexes" (Van Leene et al., 2008) [1]. Four independent biological replicates of a selected TPL-GS-TAG line were grown simultaneously, crosslinked with formaldehyde, and proteins were isolated from whole plant tissue via TAP. Purified proteins were treated with trypsin, and the peptides were analyzed via mass spectrometry. Datasets are hosted in the MassIVE public repository (reference number: MSV000082477, https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=f16255fb7080426a9fe1926b4d3d5862). The data in this article has not been published elsewhere and is original to this work.

Source: Data in Brief

DOI: 10.1016/j.dib.2018.08.059

Authors: Joe Collins, Craig Dufresne, William B. Gurley, Sixue Chen

Institutions: University of Florida, Thermo Fisher Scientific (United States)

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,220

Funders: University of Florida Foundation, University of Florida

OpenAlex ID: W2889167965

Cites: 13

Cited by:

Related to: 10