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Quantitative Proteomic Analysis Reveals Caffeine‐Perturbed Proteomic Profiles in Normal Bladder Epithelial Cells

Year: 2018

Type: article

Abstract: Lower urinary tract symptoms (LUTSs) are highly prevalent among the elderly and negatively impact quality of life. Since caffeinated beverages are enjoyed worldwide and the relationship between LUTS and caffeine is still not fully understood, it would be of particular interest to examine the underlying mechanisms that drive caffeine's influence on LUTS development and progression. The aim of this study is to characterize the effects of caffeine on hTert-immortalized normal bladder epithelial cells by investigating whether exposure to caffeine can cause potential changes in the bladder proteome and/or biological pathways. In labeled LC-MS/MS proteomic analysis, 57 proteins are found as being differentially expressed in caffeine-treated bladder epithelial cells, compared to controls; this included 32 upregulated and 25 downregulated proteins. Further functional gene enrichment analysis reveals that caffeine affects major biological pathways, including those for "muscle contraction" and "chromatin assembly." These findings provide new scientific insights that may be useful in future studies investigating the role of caffeine in bladder dysfunctions.

Source: PROTEOMICS

DOI: 10.1002/pmic.201800190

Authors: Muhammad Shahid, Minhyung Kim, Austin Yeon, Allen M. Andres, Sungyong You, Jayoung Kim

Institutions: Cedars-Sinai Medical Center, Cedars-Sinai Smidt Heart Institute, University of California, Los Angeles

Language: English

Topic: Urinary Bladder and Prostate Research

Subfield: Urology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green