Labeling and Detection of Marrow Derived Mesenchymal Stromal Cells using Magnetic Resonance Imaging
Year: 2008
Type: dissertation
Abstract: Stem cell therapies hold great promise for diseases such as stroke, where few effective\ntreatment options exist. Clinical translation of experimental stem cell therapies requires\nthe ability to monitor delivery and behaviour of cells non-invasively in-vivo with clinical\nimaging modalities such as MRI. This thesis presents the translation of established\nmethods for labelling and imaging stem cells with specialized MRI systems to a more clinically relevant setting. \nA methodology for harvesting and labelling a cell population containing stem cells\nwith iron oxide for detection with a clinical MRI system is presented and single cell\ndetection is demonstrated in-vitro. The feasibility of detecting iron oxide labelled stem cells intravenously delivered in a rat model of stroke is tested. Results demonstrate that while MRI is highly sensitive to the presence and distribution of iron oxide containing cells in-vivo the true origin of these cells remains ambiguous with the current methodology.
Source: TSpace (University of Toronto)
Author: Emidio Tarulli
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2889809608