Work

Simultaneous Cryosectioning of Multiple Rodent Brains

Year: 2018

Type: article

Abstract: Histology and immunohistochemistry are routine methods of analysis to visualize microscopic anatomy and localize proteins within biological tissue. In neuroscience, as well as a plethora of other scientific fields, these techniques are used. Immunohistochemistry can be done on slide mounted tissue or free-floating sections. Preparing slide-mounted samples is a time intensive process. The following protocol for a technique, called the Megabrain, reduced the time taken to cryosection and mount brain tissue by up to 90% by combining multiple brains into a single frozen block. Furthermore, this technique reduced variability seen between staining rounds, in a large histochemical study. The current technique has been optimized for using rodent brain tissue in downstream immunohistochemical analyses; however, it can be applied to different scientific fields that use cryosectioning.

Source: Journal of Visualized Experiments

DOI: 10.3791/58513

Authors: Tabitha R.F. Green, J. Bryce Ortiz, Jordan L. Harrison, Jonathan Lifshitz, Rachel K. Rowe

Institutions: Barrow Neurological Institute, University of Arizona, Phoenix Children's Hospital, University of Arizona College of Medicine- Phoenix, University of Bath, Phoenix VA Health Care System

Language: English

Topic: Medical and Biological Sciences

Subfield: Anatomy

Field: Medicine

Domain: Health Sciences

Open Access status: green

OpenAlex ID: W2890985691

FWCI:

Cites: 11

Cited by: 8

Related to: 10