Work

Switchable Fluorophores for Single-Molecule Localization Microscopy

Year: 2018

Type: review

Abstract: The past decade has witnessed an explosion in the use of super-resolution fluorescence microscopy methods in biology and other fields. Single-molecule localization microscopy (SMLM) is one of the most widespread of these methods and owes its success in large part to the ability to control the on-off state of fluorophores through various chemical, photochemical, or binding-unbinding mechanisms. We provide here a comprehensive overview of switchable fluorophores in SMLM including a detailed review of all major classes of SMLM fluorophores, and we also address strategies for labeling specimens, considerations for multichannel and live-cell imaging, potential pitfalls, and areas for future development.

Source: Chemical Reviews

DOI: 10.1021/acs.chemrev.7b00767

Authors: Honglin Li, Joshua C. Vaughan

Institution: University of Washington

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Burroughs Wellcome Fund, University of Washington, Wellcome Trust, National Institute of Mental Health

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