Hepatocyte‐like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers
Year: 2018
Type: article
Abstract: Direct reprogramming represents an easy technique to generate induced hepatocytes (iHeps) from somatic cells. However, current protocols are accompanied by several drawbacks as iHeps are heterogenous and lack fully mature phenotypes of primary hepatocytes. Here, we established a polycistronic expression system to induce the direct reprogramming of mouse embryonic fibroblasts towards hepatocytes. The resulting iHeps are homogenous and display key properties of primary hepatocytes, such as expression of hepatocyte markers, albumin secretion, and presence of liver transaminases. iHeps also possess the capacity to repopulate decellularized liver tissue and exhibit enhanced hepatic maturation. As such, we present a novel strategy to generate homogenous and functional iHeps for applications in tissue engineering and cell therapy.
Source: Biotechnology and Bioengineering
DOI: 10.1002/bit.26784
Institutions: Utrecht University, University Medical Center Utrecht, Hubrecht Institute for Developmental Biology and Stem Cell Research, Instituto de Investigación Sanitaria Aragón, Fundacion Agencia Aragonesa para la Investigacion y el Desarrollo, Centro de Investigación Biomédica en Red, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Investigación Sanitaria Fundación Jiménez Díaz, Universidad Carlos III de Madrid
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,060
OpenAlex ID: W2891471469