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High-resolution infrared imaging of biological samples with third-order sum-frequency generation microscopy

Year: 2018
Type: article
Abstract: We studied the use of vibrationally resonant, third-order sum-frequency generation (TSFG) for imaging of biological samples. We found that laser-scanning TSFG provides vibrationally sensitive imaging capabilities of lipid droplets and structures in sectioned tissue samples. Although the contrast is based on the infrared-activity of molecular modes, TSFG images exhibit a high lateral resolution of 0.5 µm or better. We observed that the imaging properties of TSFG resemble the imaging properties of coherent anti-Stokes Raman scattering (CARS) microscopy, offering a nonlinear infrared alternative to coherent Raman methods. TSFG microscopy holds promise as a high-resolution imaging technique in the fingerprint region where coherent Raman techniques often provide insufficient sensitivity.
DOI: 10.1364/boe.9.004807
Language: English
Subfield: Biophysics
Open Access status: gold
Estimated APC paid: $1,913
Funders: National Science Foundation, Simons Foundation, National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Biomedical Imaging and Bioengineering, Division of Chemistry
Awards: G230785495, G2318624699, G278443292, G4635388232, G6791967163, G6861457604, Rapid chemical imaging with sum-frequency generation microscopy, A Laser Microbeam Biotechnology Resource, Skin Defense by Dermal Adipocytes, Induction of fat regeneration in skin wounds by hair follicle signaling, G9000177675, G9000376361, G986754019
OpenAlex ID: W2891559605
FWCI: 2.085
Cites: 34
Cited by: 40
Related to: 10