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Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant—Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A)

Year: 2018
Type: article
Abstract: Although the majority of late-onset Alzheimer's disease (AD) patients are labeled sporadic, multiple genetic risk variants have been identified, the most powerful and prevalent of which is the e4 variant of the Apolipoprotein E (APOE) gene. Here, we generated human induced pluripotent stem cell (hiPSC) lines from the peripheral blood mononuclear cells (PBMCs) of a clinically diagnosed AD patient [ASUi005-A] and a non-demented control (NDC) patient [ASUi006-A] homozygous for the APOE4 risk allele. These hiPSCs maintained their original genotype, expressed pluripotency markers, exhibited a normal karyotype, and retained the ability to differentiate into cells representative of the three germ layers. Resource tableUnique stem cell lines identifierASUi005-AASUi006-AAlternative names of stem cell linesASU-161ASU-487InstitutionArizona State University; Tempe, AZ; USAContact information of distributorDavid Brafman, [email protected]Type of cell linesiPSCOriginHumanCell sourceHuman peripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingCytoTune®-iPS 2.0 Reprogramming SystemMultiline rationaleAge-matched Alzheimer's disease and non-demented control hiPSC lines homozygous for the APOE 4 risk factorGene modificationNoType of modificationN/AAssociated diseaseAlzheimer's diseaseGene/locusApolipoprotein E (APOE)Method of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateMarch 2018Cell line repository/bankNot applicableEthical approvalMayo Clinic Institutional Review Board; IRB # 15-008674
DOI: 10.1016/j.scr.2018.09.007
Language: English
Open Access status: gold
Estimated APC paid: $2,360
OpenAlex ID: W2891632768
FWCI: 0.235
Cites: 5
Cited by: 4
Related to: 10