Impact of Aging and HIV Infection on the Function of the C-Type Lectin Receptor MINCLE in Monocytes
Year: 2018
Type: article
Abstract: Both aging and HIV infection are associated with an enhanced pro-inflammatory environment that contributes to impaired immune responses and is mediated in part by innate immune pattern-recognition receptors. MINCLE is a C-type lectin receptor that recognizes trehalose-6,6'-dimycolate or "cord factor," the most abundant glycolipid in Mycobacterium tuberculosis. Here, we evaluated MINCLE function in monocytes in a cohort of HIV-infected and uninfected young (21-35 years) and older adults (≥60 years) via stimulation of peripheral blood mononuclear cells with trehalose-6,6-dibehenate, a synthetic analog of trehalose-6,6'-dimycolate and measurement of cytokine production (interleukin [IL]-10, IL-12, IL-6, tumor necrosis factor-α) by multicolor flow cytometry. Our studies show an age- and HIV-associated increase in cytokine multifunctionality of monocytes both at the population and single cell level that was dominated by IL-12, IL-10, and IL-6. These findings provide insight into the host response to M. tuberculosis and possible sources for the pro-inflammatory environment seen in aging and HIV infection.
Institution: Yale University
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
Awards: Research Training in Disability & Disabling Disorders, Midcareer Award in Translational Immunology of Aging, G453267231, Developing Research At The Interface Of HIV And Aging, The Impact of Aging and HIV Infection on Immunologic and Transcriptomic Signatures of Influenza Vaccine Response, The effects of age and HIV infection on C-type lectin receptor function, G9000075367, Immune signatures of vaccine responses in vulnerable populations, Yale Clinical and Translational Science Award, Training in Investigative Infectious Diseases, Claude D. Pepper Older Americans Independence Center at Yale
OpenAlex ID: W2891733376