Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS
Year: 2018
Type: editorial
Abstract: Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here, we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated direct analysis of intact proteins such as somatropin in human plasma, achieving sensitivity (0.1-1.0 fmole) and speed (1-5 min) on par with enzyme-linked immunosorbent assay (ELISA).
Source: Analytical Chemistry
Authors: Yuchao Chen, Pan Mao, Daojing Wang
Institution: Newomics (United States)
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Funders: U.S. Department of Health and Human Services, National Institutes of Health, National Institute on Aging, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Environmental Health Sciences, National Center for Complementary and Integrative Health
Awards: AD-MS Chips for Biofluids-Based Diagnosis of Alzheimer's Disease, Senescence-Chips for Radiation Biodosimetry, G1740348622, G1742132936, G2749707749, Picoelectrospray Ionization Mass Spectrometry for Top-down Proteomics, G3089326124, G3091354705, Picoelectrospray Ionization Mass Spectrometry for Top-down Proteomics, G3740951016, G4138437340, G414612196, G4597545058, G5094328609, G6778851630, G6815549504, AD-MS Chips for Biofluids-Based Diagnosis of Alzheimer's Disease, G8172376892, Microfluidic Chips for Biomonitoring
OpenAlex ID: W2892102802