miR-205 Regulates Basal Cell Identity and Stem Cell Regenerative Potential During Mammary Reconstitution
Year: 2018
Type: article
Abstract: Mammary gland development is fueled by stem cell self-renewal and differentiation. External cues from the microenvironment coupled with internal cues such as post-transcriptional regulation exerted by microRNAs regulate stem cell behavior and fate. Here, we have identified a miR-205 regulatory network required for mammary gland ductal development and stem cell regeneration following transplantation into the cleared mammary fat pad. In the postnatal mammary gland, miR-205 is predominantly expressed in the basal/stem cell enriched population. Conditional deletion of miR-205 in mammary epithelial cells impairs stem cell self-renewal and mammary regenerative potential in the in vitro mammosphere formation assay and in vivo mammary reconstitution. miR-205 null transplants display significant changes in basal cells, basement membrane, and stroma. NKD1 and PTPA, which inhibit the Wnt signaling pathway, and AMOT, which causes YAP cytoplasmic retention and inactivation were identified as miR-205 downstream mediators. These studies also confirmed that miR-205 is a direct ΔNp63 target gene that is critical for the regulation of basal cell identity. Stem Cells 2018;36:1875-15.
Source: Stem Cells
DOI: 10.1002/stem.2914
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
Funders: Baylor College of Medicine, Ministerio de Economía y Competitividad, National Institutes of Health, University of Texas MD Anderson Cancer Center, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, U.S. National Library of Medicine, National Center for Research Resources, Eunice Kennedy Shriver National Institute of Child Health and Human Development
OpenAlex ID: W2892390897