Work

Response to Comment on “DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification”

Year: 2018

Type: article

Abstract: , we repeated our analysis on sequencing runs from The Cancer Genome Atlas (TCGA) using their suggested parameters. We found signs of oxidative damage in all sequence contexts and irrespective of the sequencing date, reaffirming that DNA damage affects mutation-calling pipelines in their ability to accurately identify somatic variations.

Source: Science

DOI: 10.1126/science.aat0958

Authors: Lixin Chen, Pingfang Liu, Thomas C. Evans, Laurence M. Ettwiller

Institution: New England Biolabs (United States)

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: New England Biolabs