Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancer
Year: 2018
Type: article
Study design: Observational Study
Abstract: Objectives: To identify potential candidate proteins which are secretory in nature and present at a higher abundance in oral cancer patients with tobacco habits. Methods: Conditioned media of tobacco-treated and -untreated non-neoplastic oral keratinocytes were analyzed using iTRAQ-based mass spectrometry. Hypersecreted proteins; SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1 (SPOCK1); prosaposin (PSAP); and protein tyrosine phosphatase, receptor type S (PTPRS) were validated by enzyme-linked immunosorbent assay (ELISA) using saliva samples from oral cancer patients who are tobacco users. Results: Proteomic analysis of tobacco-treated and -untreated cells led to the identification of 2873 proteins. Among these, 378 proteins showed high abundance and 253 proteins showed low abundance (2-fold cutoff) in conditioned-media of tobacco-treated cells. ELISA-based validation showed significantly higher levels of SPOCK1, PSAP, and PTPRS in oral cancer patients with tobacco chewing habits compared to healthy controls. However, PSAP showed low specificity compared to SPOCK1 and PTPRS. Conclusions: This study indicates significantly increased levels of SPOCK1, PSAP, and PTPRS in saliva of oral cancer patients with tobacco habits. These protein biomarkers might be useful to identify tobacco users with high risk of developing oral cancers.
Authors: Vishalakshi Nanjappa, Remya Raja, Aneesha Radhakrishnan, Ankit P Jain, Keshava K Datta, Vinuth N Puttamallesh, Hitendra S Solanki, Sandip Chavan, Arun Patil, Santosh Renuse, Anu Jain, Don Mathew, Reetu Thakur, Rafael Guerrero-Preston, Bipin Nair, Samapika Routray, Neeta Mohanty, KB Linge Gowda, Ritesh Jadav, Sushmita Ghosal, Rekha V Kumar, Chaluvarayaswamy Ramesha, Vijay C Raghu, Premendu Prakash Mathur, TS Keshava Prasad, Joseph A Califano, David Sidransky, Arnab Pal, Mandakulutur S Ganesh, Jay Gopal Ray, Akhilesh Pandey, Harsha Gowda, Aditi Chatterjee
Institutions: Institute of Bioinformatics, Amrita Vishwa Vidyapeetham, Manipal Academy of Higher Education, Post Graduate Institute of Medical Education and Research, KIIT University, Johns Hopkins University, University of Puerto Rico, Medical Sciences Campus, University of Puerto Rico System, Siksha O Anusandhan University, All India Institute of Medical Sciences Bhubaneswar, Kidwai Memorial Institute of Oncology, Dr. R. Ahmed Dental College and Hospital, Pondicherry University, Yenepoya University, University of California San Diego, Moores Cancer Center, Johns Hopkins Medicine
Language: English
Subfield: Periodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $1,500
OpenAlex ID: W2893117679