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Novel Metabolic Pathways and Regulons for Hexuronate Utilization in Proteobacteria

Year: 2018
Type: article
Abstract: Hexuronate catabolic pathways and their transcriptional networks are highly variable among different bacteria. We identified novel transcriptional regulators that control the hexuronate and aldarate utilization genes in four families of proteobacteria. By regulon reconstruction and genome context analysis we identified several novel components of the common hexuronate/aldarate utilization pathways, including novel uptake transporters and catabolic enzymes. Two novel families of lactonases involved in the oxidative pathway of hexuronate catabolism were characterized. Novel transcriptional regulons were validated via in vitro binding assays and gene expression studies with Polaromonas and Ralstonia species. The reconstructed catabolic pathways are interconnected with each other metabolically and coregulated via the GguR regulons in proteobacteria.
DOI: 10.1128/jb.00431-18
Authors: Jason T. Bouvier, Natalia V. Sernova, Salehe Ghasempur, Irina A. Rodionova, Matthew W. Vetting, Nawar F. Al-Obaidi, Steven C. Almo, John A. Gerlt, Dmitry A. Rodionov
Institutions: University of Illinois Urbana-Champaign, Russian Academy of Sciences, Institute for Information Transmission Problems, University of California San Diego, Albert Einstein College of Medicine, Sanford Burnham Prebys Medical Discovery Institute
Language: English
Subfield: Genetics
Open Access status: green
OpenAlex ID: W2893480143
FWCI: 1.812
Cites: 50
Cited by: 25
Related to: 10