Work

Reversibility of Structural Rearrangements in Mononucleosomes Induced by Ionic Strength

Year: 2018

Type: article

Abstract: Using fluorescence microscopy of single particles with Förster resonance energy transfer recording, structural rearrangements that occurred in nucleosomes formed on the 603 DNA template at high ionic strength were studied. Within the range of 0.7–1.3 M KCl, large-scale changes occurred in the nucleosome structure, including the formation of at least two states differing in the degree of DNA unwrapping from the histone octamer and affecting from 13 to 35 and more pairs of nucleotides. Content of the fraction of nucleosomes with modified structure varied from 60% at 0.7 M KCl to 100% at 1.3 M KCl. Preservation of the association between core histones and DNA in the new conformational states ensured reversibility of structural changes when KCl concentration was reduced to a physiological level. Reversibility was ~100% upon transition from 0.7 M to 0.15 KCl and decreased to ~50% upon transition from 1.3 M to 0.15 KCl.

Source: Moscow University Biological Sciences Bulletin

DOI: 10.3103/s0096392518030070

Authors: A. V. Feofanov, T. V. Andreeva, V. M. Studitsky, M. P. Kirpichnikov

Institutions: Russian Academy of Sciences, Lomonosov Moscow State University, Institute of Bioorganic Chemistry, Fox Chase Cancer Center

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: Russian Science Foundation

OpenAlex ID: W2894606227

FWCI: 0.236

Cites: 16

Cited by: 4

Related to: 10