Work

Pooled functional genomic screens for intracellular calcium effectors

Year: 2018

Type: preprint

Abstract: Cytoplasmic calcium transients relay cellular signals on timescales from milliseconds to hours, and the dynamic nature of calcium signals has slowed functional genomic screening for cellular calcium effectors. Here, we present a new strategy to identify calcium handling genes via a pooled knockdown employing the calcium-sensitive photo-switchable fluorescent protein, CaMPARI. This assay, cal-Seq, enabled identification of regulators for both cellular desensitization and histamine induced calcium signaling including GPR99, a leukotriene binding receptor, with implications in asthma treatment.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.1101/434910

Authors: Anjali Rao, Joel M. Kralj

Institution: University of Colorado Boulder

Language: English

Topic: Ion Channels and Receptors

Subfield: Sensory Systems

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institute of General Medical Sciences