Superiority of SpiroZin2 Versus FluoZin-3 for monitoring vesicular Zn2+ allows tracking of lysosomal Zn2+ pools
Year: 2018
Type: article
Abstract: Small-molecule fluorescent probes are powerful and ubiquitous tools for measuring the concentration and distribution of analytes in living cells. However, accurate characterization of these analytes requires rigorous evaluation of cell-to-cell heterogeneity in fluorescence intensities and intracellular distribution of probes. In this study, we perform a parallel and systematic comparison of two small-molecule fluorescent vesicular Zn 2+ probes, FluoZin-3 AM and SpiroZin2, to evaluate each probe for measurement of vesicular Zn 2+ pools. Our results reveal that SpiroZin2 is a specific lysosomal vesicular Zn 2+ probe and affords uniform measurement of resting Zn 2+ levels at the single cell level with proper calibration. In contrast, FluoZin-3 AM produces highly variable fluorescence intensities and non-specifically localizes in the cytosol and multiple vesicular compartments. We further applied SpiroZin2 to lactating mouse mammary epithelial cells and detected a transient increase of lysosomal free Zn 2+ at 24-hour after lactation hormone treatment, which implies that lysosomes play a role in the regulation of Zn 2+ homeostasis during lactation. This study demonstrates the need for critical characterization of small-molecule fluorescent probes to define the concentration and localization of analytes in different cell populations, and reveals SpiroZin2 to be capable of reporting diverse perturbations to lysosomal Zn 2+ .
Source: Scientific Reports
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,690
Awards: Multicolor Fluorescent Sensors for Imaging Zinc Dynamics in Cells, Regulation of Cell Signaling by Transition Metal Dynamics, G268773857, G2801908532, G4219318344, G4435184153, G4723621516, G534080832, Imaging Mobile Zinc Biology, Technologies to Define and Map Novel Interorganelle Macromolecular Interactions, G8847262667
OpenAlex ID: W2895349004