Probe-intensity profiles for intron-exon boundaries (edges) of the 27 exons using 35-nucleotide probes and 30% formamide, where each color represents a single exon
Year: 2011
Type: other
Abstract: Copyright information:Taken from "Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing"Genome Biology 2003;4(10):R66-R66.Published online 19 Sep 2003PMCID:PMC328455.Copyright © 2003 Castle et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. 3' splice sites (5' exon edge); 5' splice sites (3' exon edge). These conditions allow accurate prediction of splice sites. The dashed line marks actual splice sites, by which the exons were aligned, and the small circles mark the predicted splice-site locations for each exon after convolution with the Haar wavelet (see Materials and methods for details). Although the short exon-10 sequence contains several AT-rich regions within the exon which hybridize poorly and create a dip in the intensity profile (lower magenta curve), the splice-site predictions for exon 10 are consistent with the others.
Source: Figshare
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2896057845